Journal of Biology and Medicine: Open Access
The Ionic Liquid Tolerant Evaluation Method Using Formazan Dye in Escherichia Coli
Abstract
Ionic liquids (ILs) are used in a variety of applications. Although ILs are called “green solvents,” they exhibit significant toxicity. The toxicity of ILs presumably manifests owing to their interaction with the cell membrane. Organic solvents bind to and penetrate the cell membrane and severely affect cell permeability. Therefore, there is a possibility that the tolerance to ILs could be enhanced by utilizing organic solvent tolerance mechanisms. In this article, we proposed a method for visually evaluating the toxicity of ILs to Escherichia coli using formazan dye. Furthermore, we examined whether the method could be used to evaluate the difference in ionic liquid susceptibility of E. coli transformed with an organic solvent tolerance gene marA. E. coli transformed with marA were found to be more tolerant to several ILs. It was suggested that the organic solvent tolerance mechanism effectively works for ILs.
Keywords: Ionic liquid tolerance; Organic solvent tolerance; Escherichia coli; Tetrazolium violet.
Journal Information
- Journal Name: Journal of Biology and Medicine: Open Access
- Journal Abbreviation: J Bio Med Open Access
- Journal Code: JBMOA
- Format of Publication: Online
- Frequency of Publication: Two Issue a Year
- Language: English
- Starting Year: 2020
- Subject: Biology, Medicine, Medical Sciences
- Review Process: Single-Blind Peer-Review by Referees
- Time to 1st Decision: 1 to 4 weeks from date of submission
- Time to Acceptance: 3 to 6 weeks, depending upon the required revision cycles
- Time to Publication: 1 to 2 weeks from date of final submission
- Editor-in-Chief: CHEE KONG YAP
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